It is the largest Banana Tissue Culture laboratory in the country. The Jain Tissue Culture laboratory has successfully micro-propagated and sold over 2 million ‘Grand Nain’ banana plants with outstanding success. Same conditions (disease free) can be maintained by proper crop management practices. Red banana variety requires 8 ft. x 8 ft. and plant density would be around 700/acre. Elakki Bale AB) is the most … Subsequently, the rooted banana tissue plants are released from the laboratory to a climate controlled environments such as greenhouse for acclimatization. For rooting, NAA is commonly used. There are several benefits of using tissue cultured banana plantlets when compared to regular (conventional) suckers. Planting method for tissue culture plants Bananas can be plantfed using 3 methods: (A) tissue culture plants (B) bits and (C) suckers Tissue culture plants are small plants which are grown from the tissue of the banana plant, in bags, and are ready for planting For hygiene reasons, this is the preferred method for planting bananas 1. Plant tissue culture is a collection of experimental methods of growing la rge number of isolated cells or tissues under sterile and controlled conditions. Conservation protocols for Ensete glaucum, a crop wild relative of banana, using plant tissue culture and cryopreservation techniques on seeds and zygotic embryos. 10) This requires 8 to 13 weeks to complete the primary hardening process. The Tissue culture technology is composed of 5 important steps such as Initiation, Multiplication, Shooting & rooting, Primary hardening in a greenhouse and Secondary hardening in shade net houses. 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